Human GAPDH Protein, Recombinant
產(chǎn)品貨號：PR00002HuP1
$ 詢價
規(guī)格 100μg
產(chǎn)品名稱：Human GAPDH Protein, Recombinant
純度：≥95 % as determined by SDS-PAGE
內(nèi)毒素：/
生物活性：/
序列起止：Gly2~Glu335
標簽：N-His tag
Uniprot鏈接：P04406
表達系統(tǒng)：E.coli
種屬：Human
預測 N 端：Met
預測分子量：36.74 kDa
緩沖液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
運輸方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
穩(wěn)定性 & 儲存條件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
復溶：Please refer to COA for detailed information
別稱：G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH
背景信息：Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
全稱：Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
說明書：待上傳