產(chǎn)品名稱:Anti-FLAG Antibody, Mouse Monoclonal, F1F
克隆號:F1F
抗體亞型:/
經(jīng)驗(yàn)證的應(yīng)用:WB
交叉反應(yīng):Predict reacts with: Human/Mouse/Rat
特異性:Flag-tag
免疫原:Synthetic Peptide
制備方法:Produced in mouse immunized with FLAG, and purified by antigen affinity chromatography.
來源:Monoclonal Mouse IgG
純化:Immunogen affinity purified
緩沖液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物:Unconjugated
濃度:Liquid
運(yùn)輸方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片:
Figure1.Application in WB
Western blot analysis of extracts of various lane1 10ng recombinant proteins containing the Flag Tag、lane2 50ng recombinant proteins containing the Flag Tag, using FLAG antibody (TM00975GeM20-F1F) at 1μg/mL. Secondary antibody: Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:4000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1.5min.
背景信息:/
全稱:/