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Anti-TUBE1 Antibody, Mouse Monoclonal, B3T

產(chǎn)品編號:CM01151HuM10-B3T
   
詢價

規(guī)格     100ul

說明書

產(chǎn)品名稱Anti-TUBE1 Antibody, Mouse Monoclonal, B3T
 
克隆號B3T
 
抗體亞型:/
 
經(jīng)驗證的應(yīng)用/
 
交叉反應(yīng)Predict reacts with: Human/Mouse/Rat
 
特異性:/

免疫原A synthetic peptide of human TUBE1
 
制備方法Produced in mouse immunized with TUBE1, and purified by antigen affinity chromatography.
 
來源Monoclonal Mouse IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
濃度Liquid
 
運輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:






Figure1.Western blot analysis of extracts of various lane1 rat  brain(+++)、lane2 mouse brain(+++), using TUBE1 antibody (CM01151HuM10-B3T) at 5ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 12min.










 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of monkey brain sections labelling TUBE1 with purified CM01151HuM10-B3T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 

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