国人国产免费av影院_日韩精品在线播放日韩中文字幕精品一区在线_豆奶视频成人版在线观看视频_亚洲色图自拍偷拍另类无码_字幕网最新中文资源站_欧美人禽zozk伦交_国产剧情秘 精品三级_A级黄色在线观看_中文字幕在线免费观看网址_久久成人国产夜夜

Anti-VIM Antibody, Rabbit Polyclonal

產(chǎn)品編號(hào):CP00051HuA10
   
詢價(jià)

規(guī)格    100uL  

說明書

產(chǎn)品名稱Anti-VIM Antibody, Rabbit Polyclonal

經(jīng)驗(yàn)證的應(yīng)用WB/IHC/IF/ICC
 
交叉反應(yīng)Human/Mouse
 
特異性human VIM
 
免疫原Recombinant human VIM protein, fragment Ser2~Glu 466; UniprotKB: P08670
 
制備方法Produced in rabbits immunized with human VIM, and purified by antigen affinity chromatography.

來源Polyclonal Rabbit IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
狀態(tài)Liquid
 
運(yùn)輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲(chǔ)存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:





Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using VIM antibody (PA00051HuA10) at 1μg/mL. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:100000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8 s.





Figure2.Application in ICC
Immunocytochemistry analysis of U-87MG (Human astroblastoma cell) cells labeling VIM with purified PA00051HuA10 at 5μg/mL. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti-rabbit IgG (Alexa Fluor® 488, GB25303) was used as the secondary antibody at 1/1000 (2μg/mL) dilution.  DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.


 


 


Figure3.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon  tissue sections labelling VIM with purified PA00051HuA10 at 5μg/mL. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1: 4000 dilution. PBS instead of the primary antibody was used as the negative control.



別稱CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, OTTHUMP00000019224, VIM, VIME_HUMAN, Vimentin.
 
背景信息Vimentin. Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
 
全稱Vimentin (VIM)