Anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody, Recombinant Antibody, R1
產(chǎn)品編號：RPTMB4A1
￥ 詢價
規(guī)格 100ug
產(chǎn)品名稱：Anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody, Recombinant Antibody, R1
克隆號：R1
抗體亞型：/
經(jīng)驗證的應(yīng)用：WB/IP/IHC/IF/F
交叉反應(yīng)：Human, Mouse, Rat, Hamstert, Monkey, Bovine, Dog, Pig, Yeast, Fruitfly
特異性：Recognizes Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
免疫原：Synthetic peptide
制備方法：/
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of HeLa and C6 cells, untreated or treated with the TPA(200nM, 10min), using RPTMB4A1 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
背景信息：Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
全稱：/
說明書：待上傳