Anti-Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody, Recombinant Antibody, R2
產(chǎn)品編號：RPTMB3A1-R2
￥ 詢價
規(guī)格 100ug
 |
說明書 |
產(chǎn)品名稱：Anti-Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody, Recombinant Antibody, R2
克隆號：R2
抗體亞型：/
經(jīng)驗證的應用：WB/IP/IHC/IF
交叉反應：Human, Mouse, Rat
特異性：Recognizes Acetyl-CoA Carboxylase (Ser79)
免疫原：Synthetic peptide
制備方法：/
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of HepG2 cells, untreated , starved or treated with the Oligomycin (0.5 μM, 30 min), treated with H2O2 (10mM 30min), using RPTMB3A1-R2 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:5000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
Figure2.Immunofluorescent analysis of Hela cells, treated with H2O2 (10mM 30min), using RPTMB3A1-R2 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：/
背景信息：Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids. In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues. However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
全稱：/
克隆號：R2
抗體亞型：/
經(jīng)驗證的應用：WB/IP/IHC/IF
交叉反應：Human, Mouse, Rat
特異性：Recognizes Acetyl-CoA Carboxylase (Ser79)
免疫原：Synthetic peptide
制備方法：/
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of HepG2 cells, untreated , starved or treated with the Oligomycin (0.5 μM, 30 min), treated with H2O2 (10mM 30min), using RPTMB3A1-R2 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:5000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
Figure2.Immunofluorescent analysis of Hela cells, treated with H2O2 (10mM 30min), using RPTMB3A1-R2 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：/
背景信息：Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids. In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues. However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
全稱：/