Anti-Sumo 1 Antibody, Recombinant Antibody, R4
產(chǎn)品編號：RPTMA7A1-R4
￥ 詢價
規(guī)格 100ug
濃度 1.3mg/ml
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說明書 |
產(chǎn)品名稱：Anti-Sumo 1 Antibody, Recombinant Antibody, R4
克隆號：R4
抗體亞型：Rabbit IgG
經(jīng)驗證的應(yīng)用：WB/ICC/IHC
交叉反應(yīng)：Human, Mouse, Rat
特異性：SUMO-1 Antibody detects recombinant SUMO-1 and endogenous levels of sumoylated proteins.
免疫原：Synthetic peptide
宿主：CHOS
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
狀態(tài)：Liquid
應(yīng)用：The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB: 0.5~5ug/ml ICC:5~20ug/m IHC: 5~20ug/ml
使用&儲存條件：Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.
質(zhì)量驗證圖：
Figure1.Western blot analysis of 293T and HeLa cells, untreated or treated with the MG132 (50 µM, 90 minutes), using RPTMA7A1-R4 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
Figure2.Immunofluorescent analysis of Hela cells , using RPTMA7A1-R4 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：DAP1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, GMP1, OFC10, PIC 1, PIC1, SENP2, Sentrin 1,Sentrin,Smallubiquitinrelated modifier 1, Small ubiquitin-like modifier 1, Small ubiquitin-related modifier 1, SMT3, SMT3 homolog 3,SMT3 suppressor of mif two 3 homolog 1, SMT3, yeast, homolog 3, Smt3C, SMT3H3, SUMO-1, SUMO1, SUMO1_HUMAN, Ubiquitinhomologydomainprotein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1,Ubiquitinhomologydomain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, UBL1.
背景信息：Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
全稱：/
克隆號：R4
抗體亞型：Rabbit IgG
經(jīng)驗證的應(yīng)用：WB/ICC/IHC
交叉反應(yīng)：Human, Mouse, Rat
特異性：SUMO-1 Antibody detects recombinant SUMO-1 and endogenous levels of sumoylated proteins.
免疫原：Synthetic peptide
宿主：CHOS
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
狀態(tài)：Liquid
應(yīng)用：The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB: 0.5~5ug/ml ICC:5~20ug/m IHC: 5~20ug/ml
使用&儲存條件：Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.
質(zhì)量驗證圖：
Figure1.Western blot analysis of 293T and HeLa cells, untreated or treated with the MG132 (50 µM, 90 minutes), using RPTMA7A1-R4 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
Figure2.Immunofluorescent analysis of Hela cells , using RPTMA7A1-R4 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：DAP1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, GMP1, OFC10, PIC 1, PIC1, SENP2, Sentrin 1,Sentrin,Smallubiquitinrelated modifier 1, Small ubiquitin-like modifier 1, Small ubiquitin-related modifier 1, SMT3, SMT3 homolog 3,SMT3 suppressor of mif two 3 homolog 1, SMT3, yeast, homolog 3, Smt3C, SMT3H3, SUMO-1, SUMO1, SUMO1_HUMAN, Ubiquitinhomologydomainprotein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1,Ubiquitinhomologydomain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, UBL1.
背景信息：Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
全稱：/