Anti-O-Linked N-Acetylglucosamine Antibody, Recombinant Antibody, R1
產(chǎn)品編號：RPTMC8A1-R1
￥ 詢價
規(guī)格 100ug
濃度 1.3mg/ml
產(chǎn)品名稱：Anti-O-Linked N-Acetylglucosamine Antibody, Recombinant Antibody, R1
克隆號：R1
抗體亞型：Rabbit IgG
經(jīng)驗證的應(yīng)用：WB/IP
交叉反應(yīng)：All
特異性：Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc).
免疫原：Synthetic peptide
宿主：CHOS
來源：Recombinant Rabbit IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
狀態(tài)：Liquid
應(yīng)用：The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB: 0.5~5ug/ml IP:1:20-1:50
使用&儲存條件：Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.
質(zhì)量驗證圖：
Figure1.Application in WB
Western blot analysis of 293T and HeLa cells, untreated or treated with the MG132 (50 µM, 90 minutes), using RPTMC8A1-R1 at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).
Figure2.Application in ICC
Immunofluorescent analysis of Hela cells , using RPTMC8A1-R1 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：O-GlcNAc, O糖基化
背景信息：A distinct form of protein glycosylation, beta-linked N-acetyl-glucosamine (GlcNAc) moieties can be added to serine or threonine residues of proteins. This differs from other forms of glycosylation, as it typically is a single moiety rather than the complex branched sugars that are more commonly studied. It is thought that these modifications happen in a much more dynamic cycle more reminiscent of phosphorylation modifications. GlcNAc modified proteins are found in the cytoplasm and nucleus and are modulated by means of specific O-GlcNAc transferases (OGT) as well as GlcNAcase activity that can be inhibited using the Thiamet-G (TMG) inhibitor. Mass spectrometry analysis of this modification has been complicated due to the loss of the GlcNAc group during ionization and fragmentation, but methods and technologies such as electron transfer dissociation (ETD) are opening up new avenues to study these modifications. O-GlcNAc could play an important role in many cellular processes, including metabolism, growth, morphogenesis, apoptosis, transcription, and it may play a critical role in cancer.
全稱：/
說明書：待上傳