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Anti-COX4I1 Antibody, Mouse Monoclonal, C8T

產(chǎn)品編號:MA00294HuM10-C8T
   
詢價

規(guī)格     100ul

說明書
 

產(chǎn)品名稱Anti-COX4I1 Antibody, Mouse Monoclonal, C8T
 
克隆號C8T
 
抗體亞型:/
 
經(jīng)驗證的應(yīng)用/
 
交叉反應(yīng)Predict reacts with: Human/Mouse/Rat
 
特異性:/

免疫原Recombinant human COX4I1 protein
 
制備方法Produced in mouse immunized with COX4I1, and purified by antigen affinity chromatography.
 
來源Monoclonal Mouse IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
濃度Liquid
 
運輸方式This antibody is shipped as liquid solution at ambient t
emperature. Upon receipt, store it immediately at the temperature recommended.
 
儲存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:






Figure1.Western blot analysis of extracts of various lane1 mouse heart、lane2  rat heart, using COX4I1 antibody (MA00294HuM10-C8T) at 3ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3min.










 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey brain、Monkey cerebellum、Monkey left ventricle、Monkey kidney sections labelling COX4I1 with purified MA00294HuM10-C8T at 10ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 



別稱COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
 
背景信息COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

全稱Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1)