Anti-KRT18 Antibody, Mouse Monoclonal, H1F
產(chǎn)品編號(hào)：MA00894HuM10-H1F
$ 詢價(jià)
規(guī)格 100ul
 |
說(shuō)明書(shū) |
產(chǎn)品名稱：Anti-KRT18 Antibody, Mouse Monoclonal, H1F
克隆號(hào)：H1F
抗體亞型：/
經(jīng)驗(yàn)證的應(yīng)用：/
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
特異性：/
免疫原：/
制備方法：Produced in mouse immunized with KRT18, and purified by antigen affinity chromatography.
來(lái)源：Monoclonal Mouse IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
濃度：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various lane1 MCF7(++)、lane2 K562(+)、lane3 HepG2(+)、lane4 A549(++), using KRT18 antibody (MA00894HuM10-H1F) at 1ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1.5min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer sections labelling KRT18 with purified MA00894HuM10-H1F at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18
背景信息：Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
全稱：Keratin, type I cytoskeletal 18 (KRT18)
說(shuō)明書(shū)：待上傳