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Anti-ACTA2 Antibody, Rabbit Polyclonal

產(chǎn)品編號:CP00526HuA10
   
詢價

規(guī)格     50uL  100uL  200uL  可選

產(chǎn)品名稱Anti-ACTA2 Antibody, Rabbit Polyclonal
 
經(jīng)驗證的應用:WB/ICC
 
交叉反應/
 
特異性human ACTA2
 
免疫原A synthetic peptide of  human ACTA2; UniprotKB: P62736
 
制備方法Produced in rabbits immunized with human ACTA2, and purified by antigen affinity chromatography
 
來源Polyclonal Rabbit IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
狀態(tài)Liquid
 
運輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:






Figure1.Western blot analysis of extracts of various cell lines  using ACTA2 antibody (CP00526HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 8s.








 
 
























Figure2.Western blot analysis of extracts of various cell lines  using ACTA2 antibody (CP00526HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 8s.






















 


Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine  sections labelling ACTA2 with purified CP00526HuA10 at 5ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.











 



Figure4.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas   sections labelling ACTA2 with purified CP00526HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.









 

別稱ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
 
背景信息ACTA2 (also known as 偽-smooth muscle actin or 偽-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins
 
全稱Actin, aortic smooth muscle (ACTA2)
 
說明書
說明書