Anti-AIF1 Antibody, Rabbit Polyclonal
產(chǎn)品編號：PA00042HuA20
$ 詢價
規(guī)格 50uL 100uL 200uL
產(chǎn)品名稱：Anti-AIF1 Antibody, Rabbit Polyclonal
經(jīng)驗證的應(yīng)用：WB
交叉反應(yīng)：/
特異性：human AIF1
免疫原：Recombinant human AIF1 protein, fragment Met1~Pro147; UniprotKB: P55008
制備方法：Produced in rabbits immunized with human AIF1, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various cell lines using AIFI antibody (PA00042HuA20) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 6s.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum sections labelling AIF1 with purified PA00042HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum sections labelling AIF1 with purified PA00042HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：AIF1, IRT1, Daintain, Protein G1, Allograft Inflammatory Factor 1
背景信息：AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.
全稱：Allograft inflammatory factor 1 (AIF1)
說明書：待上傳