Anti-ALOX12 Antibody, Rabbit Polyclonal
產(chǎn)品編號(hào)：PA00052HuA20
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說(shuō)明書(shū)
產(chǎn)品名稱：Anti-ALOX12 Antibody, Rabbit Polyclonal
經(jīng)驗(yàn)證的應(yīng)用：WB/IHC
交叉反應(yīng)：Human
特異性：human ALOX12
免疫原：Recombinant human ALOX12 protein, fragment Gly2~Thr405; UniprotKB: P18054
制備方法：Produced in rabbits immunized with human ALOX12, and purified by antigen affinity chromatography.
來(lái)源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using ALOX12 antibody (PA00052HuA20) at 2μg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8s.
Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart sections labelling ALOX12 with purified PA00052HuA20 at 10μg/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
Figure3.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver sections labelling ALOX12 with purified PA00052HuA20 at 10μg/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12
背景信息：/
全稱：Polyunsaturated fatty acid lipoxygenase ALOX12 (ALOX12)
經(jīng)驗(yàn)證的應(yīng)用：WB/IHC
交叉反應(yīng)：Human
特異性：human ALOX12
免疫原：Recombinant human ALOX12 protein, fragment Gly2~Thr405; UniprotKB: P18054
制備方法：Produced in rabbits immunized with human ALOX12, and purified by antigen affinity chromatography.
來(lái)源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using ALOX12 antibody (PA00052HuA20) at 2μg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8s.
Figure2.Application in IHC
Figure3.Application in IHC
別稱：LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12
背景信息：/
全稱：Polyunsaturated fatty acid lipoxygenase ALOX12 (ALOX12)