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Anti-TNF Antibody, Rabbit Polyclonal

產(chǎn)品編號(hào)：PA00069MuA10
   
$ 詢價(jià)

規(guī)格     50uL  100uL  200uL

說(shuō)明書

產(chǎn)品名稱：Anti-TNF Antibody, Rabbit Polyclonal

經(jīng)驗(yàn)證的應(yīng)用：WB/IHC
 
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
 
特異性：mouse TNF
 
免疫原：Recombinant mouse TNF protein, fragment Leu80~Leu235; UniprotKB: P06804
 
制備方法：Produced in rabbits immunized with mouse TNF, and purified by antigen affinity chromatography.

來(lái)源：Polyclonal Rabbit IgG
 
純化：Immunogen affinity purified
 
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物：Unconjugated
 
狀態(tài)：Liquid
 
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片：






Figure1.Application in WB
Western blot analysis of extracts of various cell lines, using TNF antibody (PA00069MuA10) at 1μg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5 s.







Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen sections labelling TNF with purified PA00069MuA10 at 10μg/mL. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.




別稱：DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

背景信息：TNF-alpha. Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Bovine TNF-alpha  consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD) (3). Within the ECD, bovine TNF-alpha  shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNF-alpha. TNF-alpha  is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha  is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha  can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha  by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha  extracellular domain (6-8). TNF-alpha  binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha  with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha  (15).

全稱：Tumor necrosis factor (TNF)