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Anti-IL1B Antibody, Rabbit Polyclonal

產(chǎn)品編號(hào)：PA00080MuA10
   
$ 詢價(jià)

規(guī)格     50uL  100uL  200uL


說明書

產(chǎn)品名稱：Anti-IL1B Antibody, Rabbit Polyclonal

經(jīng)驗(yàn)證的應(yīng)用：WB/IHC
 
交叉反應(yīng)：Human/Mouse
 
特異性：mouse IL1B
 
免疫原：Recombinant mouse IL1B protein, fragment Val118~Ser269; UniprotKB: P10749
 
制備方法：Produced in rabbits immunized with mouse IL1B, and purified by antigen affinity chromatography.

來源：Polyclonal Rabbit IgG
 
純化：Immunogen affinity purified
 
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物：Unconjugated
 
狀態(tài)：Liquid
 
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片：






Figure1.Application in WB
Western blot analysis of extracts of various cell lines, using IL1B antibody (PA00080MuA10-S) at 1μg/mL. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 6 s.






 




Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung  sections labelling IL1B with purified PA00080MuA10-S at 10μg/mL. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.




別稱：IL1-B, IL1-Beta, IL1F2, IL-1β, Interleukin-1 Family Member 2, Catabolin
 
背景信息：IL-1 beta/IL-1F2. IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha  (IL-1F1) and IL-1 beta  (IL-1F2), which are the products of distinct genes. IL-1 alpha  and IL-1 beta  are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha  and IL-1 beta  are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha  or IL-1 beta  and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta  but functions as a decoy receptor and negative regulator of IL-1 beta  activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha  and IL-1 beta  from interacting with IL-1 RI (1 - 4). The equine IL-1 beta  cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5 - 7). An alternatively spliced form of equine IL-1 beta  has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta  shares 65% - 75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.
 
全稱：Interleukin-1 beta (IL1B)