產(chǎn)品名稱:Anti-KRT19 Antibody, Rabbit Polyclonal
經(jīng)驗證的應(yīng)用:WB
交叉反應(yīng):Predict reacts with: Human/Mouse/Rat
特異性:human KRT19
免疫原:Recombinant human KRT19 protein, fragment Tyr127~Asp322; UniprotKB: P08727
制備方法:Produced in rabbits immunized with human KRT19, and purified by antigen affinity chromatography.
來源:Polyclonal Rabbit IgG
純化:Immunogen affinity purified
緩沖液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物:Unconjugated
狀態(tài):Liquid
運輸方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片:
Figure 1. Application in WB
Western blot analysis of extracts of various cell lines and tissues, using KRT19 antibody (PA00104HuA10) at 1μg/mL. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30 s.
別稱:CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
背景信息:Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.
全稱:Keratin, type I cytoskeletal 19 (KRT19)