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Anti-PCNA Antibody, Rabbit Polyclonal

產(chǎn)品編號(hào):CP00140HuA10
   
詢價(jià)

規(guī)格     50uL  100uL  200uL

說明書
 

產(chǎn)品名稱Anti-PCNA Antibody, Rabbit Polyclonal

經(jīng)驗(yàn)證的應(yīng)用WB/ICC
 
交叉反應(yīng)/
 
特異性human PCNA
 
免疫原Recombinant human PCNA protein, fragment Met1~Ser261; UniprotKB: P12004
 
制備方法Produced in rabbits immunized with human PCNA, and purified by antigen affinity chromatography.

來源Polyclonal Rabbit IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
狀態(tài)Liquid
 
運(yùn)輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲(chǔ)存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:






Figure1.Western blot analysis of extracts of various cell lines  using PCNA antibody (CP00140HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s







 














 












Figure2.Western blot analysis of extracts of various cell lines  using PCNA antibody (CP00140HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3s
 
















Figure3.Immunocytochemistry analysis of Huh7 (Human Hepatoma cells) cells labeling PCNA with purified CP00140HuA10 at 5 µg/mL. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100.Cells were counterstained with CY3 conjugated Anti-mouse IgG(H+L) (GB21301) 1/200 (2.5 µg/mL). Goat anti-mouse IgG (Alexa Fluor® 488, GB25301) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
 











Figure4.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Canine breast cancer tissue sections labelling PCNA with purified PA00140HuA10  at 5 ug/ml . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control. 





 
別稱Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA_HUMAN, PCNAR, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen.
 
背景信息Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3-5 exonuclease and 3-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.  This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
 
全稱Proliferating Cell Nuclear Antigen (PCNA)